EVALUATION OF THE RADICULAR INTRADENTINAL PENETRABILITY OF A BIOCERAMIC SEALER AFTER THREE FINAL IRRIGATION PROTOCOLS.

Abstract

This study evaluated the occurrence of radicular intradentinal penetration of a ready-to-use bioceramic sealer, with three chemical substances (sodium hypochlorite, saline solution and deionized water) as irrigants preceding a final irrigation with phosphate buffer solution. Thirty distobuccal roots of maxillary molars were selected and digitally radiographed. Canals were instrumented with the reciprocating system X1 Blue #25.06 and #40.06 (MK Life, Porto Alegre, Brazil). Specimens were randomly allocated into three groups according to the irrigation protocol associated with passive ultrasonic activation (PUA): G- NaOCl (2.5% sodium hypochlorite + PUA), G-SS (0.9% saline solution irrigation + PUA) and G-H20 (irrigation with deionized water + PUA). All specimens were subjected to a phosphate buffer solution irrigation. Canals were filled with a single-cone technique and bioceramic sealer (Sealer Plus BC – MK Life) mixed with a specific fluorophore for calcium compounds. The roots were transversally sectioned and each root third was prepared for Confocal Laser Scanning Microscopy (CLSM). Images obtained in CLSM were analyzed for sealer penetration in the dentinal tubules. Regardless of the final irrigation protocol tested in this study, it was not possible to observe the penetration of the bioceramic sealer in the dentinal tubules in any root third of the studied groups. It is inferred that the intradentinal penetration of this bioceramic sealer may be related to multifactorial conditions that may interfere in the material properties.