Evaluation of the resistance and modulus of elasticity of mineralized and demineralized bone for the test of microtensile

Abstract

Regeneration is made through bone grafts that be one scaffold for the tecidual repairing, increasing bone tissue in the resultant defects, filling the alveolous after extraction to preserve the height and thickness of the alveolar rigde. These procedures are carried through the use of bone grafts. With the development of the osseointegrated implants, it was seen necessity to keep a good quantities and quality bone. The aim is evaluate the resistance the traction and modulus of elasticity of the mineralized and demineralized bone of calvarial of rats to determine initial standards (maximum and minimum) for comparisons with possible biomaterials of fulfilling of osteoinduction and osteoconduction. The 24 animals divided in 2 groups had been used. Group modulus of elasticity being that we will use the two parietals of the animal, having made possible 24 specimens, subdivided in mineralized (n=12) and demineralized (n=12). Group microtensile, being that 12 hemi-calvarial had been used for the demineralized sub-group and the others 12 hemi-calvarial for the mineralized sub-group. After the death of the animals, the specimens will be collected and treated in accordance with the sub-group (mineralized and demineralized). The tests had been carried through in a machine of universal test. The resistance averages the tensile of the mineralized one was mineralized 129,814 ± 34.921 MPa and the demineralized one was 18,547 ± 3,682 MPa. For the modulus of elasticity, the values of 1377,792 ± 208.331 MPa for the mineralized group and 49,669 ± 11,204 MPa for the demineralized group, when comparing the resistance and modulus of elasticity the tensile between the demineralized and mineralized sub-group observe a estatistical significant difference for the mineralized group (p<0,001). Concluded that the microtensile test can be used to evaluate the mechanical properties in this biological experimental model.